Enzyme activity profiles and ELISA analysis of biomarkers from human saliva and gingival crevicular fluid during orthodontic tooth movement using self-ligating brackets.

AIM Profiles of orthodontic tooth movement biomarkers, i.e., Lactate Dehydrogenase (LDH), Aspartate Aminotransferase (AST), Tartrate-resistant Acid Phosphatase (TRAP) and Alkaline Phosphatase (ALP), using Self-ligating Brackets (SLBs) and possible relationships among their activities and total enzymes produced were determined. METHODS Saliva and Gingival Crevicular Fluid (GCF) were collected from 19 subjects (n=19) before and during orthodontic treatment (5 weeks). The subjects were bonded with SLBs with 100 g or 150 g of orthodontic force. Enzyme assays, ELISA and tooth movement measurements were performed. RESULTS A statistical analysis (paired t-test) showed that compared to baseline values, significant differences (p<0.05) were observed in the saliva levels of AST at week 5, the levels of TRAP at week 2, and the levels of ALP at weeks 1 to 5. In the GCF, LDH showed significant differences (p<0.05) at weeks 2, 3 and 4 (100 g) and at weeks 1, 2 and 3 (150 g). AST showed significant differences (p<0.05) at weeks 4 and 5 (100 g) and at weeks 3 and 4 (150 g), while TRAP exhibited a significant difference at week 5 (100 g). Pearson's correlation test revealed a weak correlation between enzyme activities and total enzymes. The use of 100 g compared to 150 g of force for tooth movement was not significant (p>0.05). CONCLUSION Therefore, 100 g is recommended as a better force for patient comfort. AST, TRAP and ALP in the saliva and LDH, AST and TRAP in the GCF are potential biomarkers in orthodontic tooth movement using SLB systems.